Quantity 125m g (1.25 ml)

Store at -20°C

Description 

Zeocin is a formulation of phleomycin D1, a basic, water-soluble, copperchelated glycopeptide isolated from Streptomyces verticillus and shows strong toxicity against bacteria, fungi (including yeast), plants, and mammalian cell lines. The blue color of the solution is due to the presence of copper and the copper-chelated form of Zeocin is inactive. When the antibiotic enters the cell, the copper cation is reduced from Cu2+ to Cu+ and removed by sulfhydryl compounds in the cell. Upon copper removal, Zeocin is activated, and binds and cleaves DNA, causing cell death. A Zeocin resistance protein of 13,665 Da, has been isolated and characterized. The protein is the product of the Sh ble gene (Streptoalloteichus hindustanus bleomycin gene), binds stoichiometrically to Zeocin and inhibits its DNA strand cleavage activity. Expression of this protein in eukaryotic and prokaryotic hosts confers resistance to Zeocin.

Specifications

Contents:  100 mg/ml solution in deionized, autoclaved water.

Shipping/Storage:Shipped on blue ice. Store at -20°C.

E. coli Selection: 25-50 μg/ml in low salt LB medium (NaCl concentration should not exceed 5 g/liter.)

Yeast Selection:50-300 μg/ml in YPD or minimal medium

Mammalian Cells:50-1000 μg/ml in suitable medium (varies with cell Selection: line).

Handling Zeocin

? Always wear gloves, a laboratory coat, and safety glasses when handling Zeocin containing solutions. 

? Zeocin is light sensitive. Store the antibiotic and plates or medium containing the antibiotic in the dark. 

? Reduce the salt in bacterial medium and adjust the pH to 7.5 to keep Zeocin active as high ionic strength and acidity or basicity inhibit Zeocin activity. 

? Store Zeocin at -20℃ and thaw on ice before use.