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首頁>>免疫學>>一抗>>磷酸化絲氨酸/蘇氨酸激酶p90RSK蛋白重組兔單克隆抗體
磷酸化絲氨酸/蘇氨酸激酶p90RSK蛋白重組兔單克隆抗體
  • 產(chǎn)品貨號：
    BN42103R
  • 中文名稱：
    磷酸化絲氨酸/蘇氨酸激酶p90RSK蛋白重組兔單克隆抗體
  • 英文名稱：
    Rabbit anti-Phospho-RPS6KA1 (Ser380) Monoclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產(chǎn)品規(guī)格

    售價

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  • BN42103R-50ul

    50ul

    ￥2020.00

    交叉反應(yīng)：Human,Rat(predicted:Mouse) 推薦應(yīng)用：WB,IHC-P,ICC

  • BN42103R-100ul

    100ul

    ￥3240.00

    交叉反應(yīng)：Human,Rat(predicted:Mouse) 推薦應(yīng)用：WB,IHC-P,ICC

產(chǎn)品描述

英文名稱Phospho-RPS6KA1 (Ser380)
中文名稱磷酸化絲氨酸/蘇氨酸激酶p90RSK蛋白重組兔單克隆抗體
別    名RSK1(S380); RPS6KA1 (Phospho Ser380); RPS6KA1 (Phospho S380); p90 RSK1; p90rsk; p90RSK; p90S6K; pp90RSK1; Ribosomal protein S6 kinase 90kD 1; Ribosomal protein S6 kinase 90kD polypeptide 1; Ribosomal protein S6 kinase 90kDa polypeptide 1; Ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase polypeptide 1; Ribosomal S6 kinase 1; RPS6K1 alpha; rps6ka; Rps6ka1; RSK 1; RSK 1 p90; RSK; RSK1; S6K alpha 1; 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1; dJ590P13.1; EC 2.7.11.1; HU 1; HU1; MAP kinase activated protein kinase 1a; MAPKAP kinase 1A; MAPKAPK1A; MGC79981; Mitogen-activated protein kinase-activated protein kinase 1A; OTTHUMP00000004113.   
產(chǎn)品類型磷酸化抗體 
研究領(lǐng)域腫瘤  免疫學  信號轉(zhuǎn)導(dǎo)  細胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  激酶和磷酸酶  
抗體來源Rabbit
克隆類型Monoclonal
克 隆 號7A12
交叉反應(yīng)Human, Rat,  (predicted: Mouse, )
產(chǎn)品應(yīng)用WB=1:1000-5000 IHC-P=1:100-500 ICC=1:50 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量81kDa
細胞定位細胞核 細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human p90RSK around the phosphorylation site of Ser380:GF(p-S)FV 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹Rsk1 is a member of a family of 90kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c Fos and CREB.

Function:
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression.

Subunit:
Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation. Interacts with ETV1/ER81 and FGFR1.

Subcellular Location:
Nucleus. Cytoplasm.

Post-translational modifications:
Activated by phosphorylation at Ser-221 by PDPK1. Autophosphorylated on Ser-380, as part of the activation process. May be phosphorylated at Thr-359 and Ser-363 by MAPK1/ERK2 and MAPK3/ERK1.
N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.

SWISS:
Q15418

Gene ID:
6195

Database links:

Entrez Gene: 6195 Human

Entrez Gene: 20111 Mouse

Entrez Gene: 81771 Rat

Omim: 601684 Human

SwissProt: Q15418 Human

SwissProt: P18653 Mouse

SwissProt: Q63531 Rat

Unigene: 149957 Human

Unigene: 301827 Mouse

Unigene: 34915 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.