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P選擇素糖蛋白配體1抗體
  • 產品貨號：
    BN41806R
  • 中文名稱：
    P選擇素糖蛋白配體1抗體
  • 英文名稱：
    Rabbit anti-CD162 Polyclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產品規(guī)格

    售價

    備注

  • BN41806R-50ul

    50ul

    ￥1486.00

    交叉反應：Human 推薦應用：WB,ICC,Flow-Cyt,ELISA

  • BN41806R-100ul

    100ul

    ￥2360.00

    交叉反應：Human 推薦應用：WB,ICC,Flow-Cyt,ELISA

  • BN41806R-200ul

    200ul

    ￥3490.00

    交叉反應：Human 推薦應用：WB,ICC,Flow-Cyt,ELISA

產品描述

英文名稱CD162
中文名稱P選擇素糖蛋白配體1抗體
別    名CD 162; CD162; CD162 antigen; CLA; Cutaneous lymphocyte associated associated antigen; P selectin glycoprotein ligand 1; P selectin glycoprotein ligand 1 precursor; P-selectin glycoprotein ligand 1; PSGL 1; PSGL-1; PSGL1; Selectin P ligand; SELPL_HUMAN; SELPLG.  
研究領域細胞生物  免疫學  細胞粘附分子  細胞表面分子  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human, 
產品應用WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg /test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量41kDa
細胞定位細胞膜 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human PSGL-1:251-350/412 <Extracellular>
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產品介紹This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011].

Function:
A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Subunit:
Homodimer; disulfide-linked. Interaction with P-, E- and L-selectins, through their lectin/EGF domains, is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Sulfation on Tyr-51 of PSGL1 is most important for high affinity L-selectin/SELL binding while P-selectin/SELP requires sulfation on Tyr-48. E-selectin/SELE binds with much lower affinity and requires the sLe(x) epitope, but apparantly not tyrosine sulfation. Dimerization appears not to be required for P-selectin/SELP binding. Interacts with SNX20. Interacts with MSN and SYK; mediates the activation of SYK by SELPLG.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Expressed on neutrophils, monocytes and most lymphocytes.

Post-translational modifications:
Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.

DISEASE:
Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

Similarity:
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 8 Sushi (CCP/SCR) domains.

SWISS:
Q14242

Gene ID:
6404

Database links:

Entrez Gene: 6404 Human

Entrez Gene: 363930 Rat

Omim: 600738 Human

SwissProt: Q14242 Human

Unigene: 591014 Human

Unigene: 215585 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PSGL-1廣泛分布于免疫細胞表面，在生物體內可與P、L、E選擇素結合。在炎癥發(fā)生、發(fā)展中有重要作用