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蛋白激酶R/EIF2AK1抗體
  • 產(chǎn)品貨號：
    BN41721R
  • 中文名稱：
    蛋白激酶R/EIF2AK1抗體
  • 英文名稱：
    Rabbit anti-PKR Polyclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產(chǎn)品規(guī)格

    售價

    備注

  • BN41721R-50ul

    50ul

    ￥1486.00

    交叉反應：Human,Mouse,Rat 推薦應用：WB,IHC-P,IHC-F,IF,ELISA

  • BN41721R-100ul

    100ul

    ￥2360.00

    交叉反應：Human,Mouse,Rat 推薦應用：WB,IHC-P,IHC-F,IF,ELISA

  • BN41721R-200ul

    200ul

    ￥3490.00

    交叉反應：Human,Mouse,Rat 推薦應用：WB,IHC-P,IHC-F,IF,ELISA

產(chǎn)品描述

英文名稱EIF2AK2 (PKR)
中文名稱干擾素誘導的雙鏈RNA活化蛋白激酶抗體
別    名double-stranded RNA-dependent Protein Kinase; interferon-induced, double-stranded RNA-activated protein kinase isoform a; protein kinase, interferon-inducible double stranded RNA dependent; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; protein kinase RNA-activated; interferon-inducible RNA-dependent protein kinase; EIF2AK1; MGC126524; PKR; PRKRv; E2AK2_HUMAN.  




研究領域細胞生物  信號轉(zhuǎn)導  細胞凋亡  生長因子和激素  激酶和磷酸酶  細胞分化  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human, Mouse, Rat, 
產(chǎn)品應用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量61kDa
細胞定位細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human PKR:251-360/551 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹PKR is an interferon-inducible serine/threonine specific protein kinase. It is widely expressed in eukaryotic organisms and activated by double stranded RNA. Activation of PKR by dsRNAs leads to autophosphorylation at multiple sites. Phosphorylation of Thr446 and Thr451 in the PKR activation loop is required in vivo and in vitro for high level kinase activity. PKR phosphorylates its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor 2 (EIF2 alpha), leading to the inhibition of protein synthesis. PKR is also involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, and also activates IKK and NFKB, thereby suppressing apoptosis. Recently, it has been reported that PKR also phosphorylates human p53 on serine 392. PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease. Alzheimer cases show prominent PKR activation in association with neuritic plaques and pyramidal neurons in the hippocampus and neocortex.

Function:
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Subunit:
Homodimer. Interacts with STRBP. Interacts with DNAJC3. Inhibited by direct interaction with viral proteins such as HCV E2, HCV NS5A and influenza A NS1. Activated by the interaction with HIV-1 Tat. Forms a complex with FANCA, FANCC, FANCG and HSP70.

Post-translational modifications:
Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 protein kinase domain.

SWISS:
P19525

Gene ID:
5610

Database links:

Entrez Gene: 5610 Human

Omim: 176871 Human

SwissProt: P19525 Human

Unigene: 131431 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

蛋白激酶R/雙鏈RNA依賴蛋白激酶（PKR）是一種干擾素誘導的、雙鏈RNA激活的絲氨酸/蘇氨酸激酶, PKR在信號轉(zhuǎn)導、細胞生長、分化和凋亡的控制中起重要作用.也有人認為：PKR是一個重要的凋亡效應物,是許多不同刺激物誘導凋亡的一個轉(zhuǎn)導物。