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X射線修復交叉互補蛋白4抗體
  • 產(chǎn)品貨號：
    BN41334R
  • 中文名稱：
    X射線修復交叉互補蛋白4抗體
  • 英文名稱：
    Rabbit anti-XRCC4 Polyclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產(chǎn)品規(guī)格

    售價

    備注

  • BN41334R-100ul

    100ul

    ￥2360.00

    交叉反應：Human(predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit) 推薦應用：WB,ELISA

  • BN41334R-200ul

    200ul

    ￥3490.00

    交叉反應：Human(predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit) 推薦應用：WB,ELISA

產(chǎn)品描述

英文名稱XRCC4
中文名稱X射線修復交叉互補蛋白4抗體
別    名DNA double strand break repair and V(D)J recombination protein XRCC4; DNA double-strand break repair and V(D)J recombination protein XRCC4; DNA repair protein XRCC4; DNA-repair protein XRCC4; X ray repair complementing defective repair in Chinese hamster cells 4 ; X ray repair cross complementing protein 4; X-ray repair complementing defective repair in Chinese hamster cells 4 ; X-ray repair cross-complementing protein 4; XRCC 4; XRCC-4; XRCC4_HUMAN.  
研究領(lǐng)域細胞生物  免疫學  信號轉(zhuǎn)導  表觀遺傳學  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human,  (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, )
產(chǎn)品應用WB=1:500-2000 ELISA=1:5000-10000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量38/60kDa
細胞定位細胞核 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human XRCC4:151-260/336 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.

Function:
Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Subunit:
Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (XRCC6/XRCC5) and PRKDC. Seems to interact directly with PRKDC but not with the Ku p70/86 dimer. Interacts with XLF/Cernunnos. Interacts with APTX and APLF.

Subcellular Location:
Nucleus.

Tissue Specificity:
Widely expressed.

Post-translational modifications:
Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
Monoubiquitinated.
Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.

Similarity:
Belongs to the XRCC4 family.

SWISS:
Q13426

Gene ID:
7518

Database links:

Entrez Gene: 7518 Human

Omim: 194363 Human

SwissProt: Q13426 Human

Unigene: 567359 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.