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蛋白酪氨酸磷酸酶-1B抗體
  • 產(chǎn)品貨號：
    BN41128R
  • 中文名稱：
    蛋白酪氨酸磷酸酶-1B抗體
  • 英文名稱：
    Rabbit anti-PTPN1 Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN41128R-100ul

    100ul

    ￥2360.00

    交叉反應(yīng)：Human,Mouse,Rat 推薦應(yīng)用：WB,IHC-P,ICC

產(chǎn)品描述

英文名稱PTPN1
中文名稱蛋白酪氨酸磷酸酶-1B抗體
別    名PTP-1B; Non receptor tyrosine phosphatase 1; Protein tyrosine phosphatase 1B; Protein-tyrosine phosphatase 1B; Protein tyrosine phosphatase non receptor type 1; Protein tyrosine phosphatase placental; PTP 1B; PTPN 1; PTPN1; Tyrosine protein phosphatase non receptor type 1; PTP-HA2; Protein-tyrosine phosphatase HA2; PTN1_HUMAN.  
研究領(lǐng)域細胞生物  神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  激酶和磷酸酶  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Human, Mouse, Rat, 
產(chǎn)品應(yīng)用WB=1:500-2000 IHC-P=1:50-200 ICC=1:100 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量48kDa
細胞定位細胞漿 細胞膜 
性    狀Liquid
濃    度1mg/ml
免 疫 原Recombinant human PTPN1:281-435/435 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
產(chǎn)品介紹PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and is a drug discovery target in the fields of diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.
PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity.

Function:
Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion

Subunit:
Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function.

Subcellular Location:
Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=Interacts with EPHA3 at the cell membrane.

Tissue Specificity:
Most abundant in testis. Also found in kidney, spleen, muscle, liver, heart and brain.

Post-translational modifications:
Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.
S-nitrosylation of Cys-215 inactivates the enzyme activity.
Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.

SWISS:
P18031

Gene ID:
5770

Database links:

Entrez Gene: 5770 Human

Entrez Gene: 19246 Mouse

Entrez Gene: 24697 Rat

Omim: 176885 Human

SwissProt: P18031 Human

SwissProt: P35821 Mouse

SwissProt: P20417 Rat

Unigene: 417549 Human

Unigene: 277916 Mouse

Unigene: 11317 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

蛋白酪氨酸磷酸酶1B（PTP1B）是一種胰島素信號的負性調(diào)節(jié)因子，與肥胖癥和2型糖尿病的發(fā)病及發(fā)展關(guān)系密切。對P-tyr基因敲除小鼠的研究表明，缺失PTP1B小鼠的胰島素敏感性明顯增加，并對肥胖有一定的抵抗作用，而另一些對動物和人體的研究結(jié)果卻與此相反，發(fā)現(xiàn)PTP1B可通過引起胰島素抵抗、瘦素抵抗及影響脂代謝等而導(dǎo)致肥胖癥和2型糖尿病的發(fā)生。一些特異性的Ptyr小分子抑制劑已經(jīng)開發(fā)并用于肥胖和糖尿病的治療中。