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磷酸化波形蛋白抗體
  • 產品貨號：
    BN40928R
  • 中文名稱：
    磷酸化波形蛋白抗體
  • 英文名稱：
    Rabbit anti-Phospho-Vimentin (Ser56) Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN40928R-100ul

    100ul

    ￥2470.00

    交叉反應：Human,Mouse(predicted:Rat,Dog,Pig,Cow,Sheep,GuineaPig) 推薦應用：WB,Flow-Cyt,ELISA

產品描述

英文名稱Phospho-Vimentin (Ser56)
中文名稱磷酸化波形蛋白抗體
別    名Vimentin (phospho S56); p-Vimentin (phospho S56); FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.  
產品類型磷酸化抗體 
研究領域腫瘤  細胞生物  免疫學  發(fā)育生物學  神經生物學  信號轉導  干細胞  細胞骨架  腫瘤細胞生物標志物  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Sheep, Guinea Pig, )
產品應用WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=0.2μg /Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量51kDa
細胞定位細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser56:S(p-S)PG 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產品介紹Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Function:
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Subunit:
Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain).Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Highly expressed in fibroblasts, somev expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Post-translational modifications:
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.

Similarity:
Belongs to the intermediate filament family.

SWISS:
P08670

Gene ID:
7431

Database links:

Entrez Gene: 7431 Human

Entrez Gene: 22352 Mouse

Entrez Gene: 81818 Rat

Omim: 193060 Human

SwissProt: P08670 Human

SwissProt: P20152 Mouse

SwissProt: P31000 Rat

Unigene: 455493 Human

Unigene: 691131 Human

Unigene: 268000 Mouse

Unigene: 2710 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Vimentin—波形蛋白。是五種主要的中間絲之一，存在于各種正常和病理性間質來源的組織，如纖維母細胞、內皮細胞、淋巴細胞等正常細胞和肉瘤、淋巴瘤、黑色素瘤等腫瘤。波形蛋白是負責維持細胞骨架完整性的蛋白之一。
























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