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磷酸化染色體濃縮調控蛋白1抗體
  • 產品貨號：
    BN40905R
  • 中文名稱：
    磷酸化染色體濃縮調控蛋白1抗體
  • 英文名稱：
    Rabbit anti-Phospho-RCC1 (Ser11) Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN40905R-100ul

    100ul

    ￥2470.00

    交叉反應：Human(predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Sheep) 推薦應用：WB,IHC-P,IHC-F,IF,ELISA

產品描述

英文名稱Phospho-RCC1 (Ser11)
中文名稱磷酸化染色體濃縮調控蛋白1抗體
別    名Cell cycle regulatory protein; CHC 1; CHC1; Chromosome condensation 1; Chromosome condensation protein 1; Guanine nucleotide releasing protein; HERC2; Ran GEF; RanGEF; RCC 1; RCC1 I; Regulator of chromosome condensation 1; Regulator of chromosome condensation; SHEP1; RCC1_HUMAN.  
產品類型磷酸化抗體 
研究領域細胞生物  染色質和核信號  信號轉導  細胞凋亡  轉錄調節(jié)因子  激酶和磷酸酶  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human,  (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, )
產品應用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量45kDa
細胞定位細胞核 細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human RCC1 around the phosphorylation site of Ser11:RR(p-S)PP 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產品介紹Ran GTPase plays important roles in nucleocytoplasmic transport in interphase and in both spindle formation and nuclear envelope (NE) assembly during mitosis. The latter functions rely on the presence of high local concentrations of GTP bound Ran near mitotic chromatin. RanGTP localization has been proposed to result from the association of Ran's GDP/GTP exchange factor, RCC1, with chromatin , but Ran is shown here to bind directly to chromatin in two modes, either dependent or independent of RCC1, and, where bound, to increase the affinity of chromatin for NE membranes.

Function:
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.

Subunit:
Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.

Subcellular Location:
Nucleus. Cytoplasm. Note=Becomes dispersed throughout the cytoplasm during mitosis.

Post-translational modifications:
N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.

Similarity:
Contains 7 RCC1 repeats.

SWISS:
P18754

Gene ID:
1104

Database links:

Entrez Gene: 1104 Human

Entrez Gene: 100088 Mouse

Entrez Gene: 682908 Rat

Omim: 179710 Human

SwissProt: P18754 Human

SwissProt: Q8VE37 Mouse

Unigene: 469723 Human

Unigene: 255045 Mouse

Unigene: 217123 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

















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