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磷酸化DNA損傷關(guān)鍵蛋白Mre11抗體
  • 產(chǎn)品貨號：
    BN40893R
  • 中文名稱：
    磷酸化DNA損傷關(guān)鍵蛋白Mre11抗體
  • 英文名稱：
    Rabbit anti-Phospho-Mre11 (Ser676) Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN40893R-100ul

    100ul

    ￥2470.00

    交叉反應(yīng)：nan(predicted:Mouse,Rat,Dog) 推薦應(yīng)用：WB,ELISA

產(chǎn)品描述

英文名稱Phospho-Mre11 (Ser676)
中文名稱磷酸化DNA損傷關(guān)鍵蛋白Mre11抗體
別    名Mre 11; MRE 11a; MRE 11b; MRE11 homolog 1; MRE11 meiotic recombination 11 homolog A; MRE11a; MRE11b; AT like disease; Ataxia telangiectasia disorder like; Ataxia-telangiectasia disorder-like; ATLD; DNA recombination and repair protein; Double strand break repair protein MRE11A; Double-strand break repair protein MRE11A; endo/exonuclease Mre11. HNGS1; meiotic recombination (S. cerevisiae) 11 homolog A; meiotic recombination 11 homolog A (S. cerevisiae); meiotic recombination 11 homolog A; MmMRE11A.MRE11_RAT  
產(chǎn)品類型磷酸化抗體 
研究領(lǐng)域腫瘤  細(xì)胞生物  免疫學(xué)  染色質(zhì)和核信號  細(xì)胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)(predicted: Mouse, Rat, Dog, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量80kDa
細(xì)胞定位細(xì)胞核 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from rat Mre11 around the phosphorylation site of Ser676:SQ(p-S)Q 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Function:
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation

Subunit:
Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo.

Subcellular Location:
Nucleus. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

DISEASE:
Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.

Similarity:
Belongs to the MRE11/RAD32 family.

SWISS:
P49959

Gene ID:
64046

Database links:

Entrez Gene: 4361 Human

Entrez Gene: 17535 Mouse

Entrez Gene: 64046 Rat

Omim: 600814 Human

SwissProt: P49959 Human

SwissProt: Q61216 Mouse

SwissProt: Q9JIM0 Rat

Unigene: 192649 Human

Unigene: 149071 Mouse

Unigene: 209040 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

    在細(xì)胞中，有多種蛋白參與DNA損傷應(yīng)答，DNA損傷是可引起癌變的細(xì)胞變化，這些蛋白在細(xì)胞發(fā)生損傷后會啟動細(xì)胞修復(fù)過程，幫助受損的細(xì)胞恢復(fù)正常。
    正常情況下，細(xì)胞會經(jīng)歷生長、分化和自然死亡的歷程。當(dāng)細(xì)胞受到損傷時，如;輻射損傷或是毒物刺激，一種多蛋白復(fù)合物參與的步驟將被啟動，進(jìn)行細(xì)胞修復(fù)工作并激活其他的生物過程。在這過程中，存在一種MRN復(fù)合物，由Mre11，Rad50和NBS1蛋白組成，MRN探測DNA損傷（DNA雙鏈?zhǔn)欠駭嗔眩┑那闆r。復(fù)合物在探測到DNA損傷信號后將把這個信息傳遞給一種酶，ATM（ataxia-telangiectasia mutated）檢測激酶（checkpoint kinase）。ATM激酶能對DNA雙鏈斷裂產(chǎn)生應(yīng)答反應(yīng)，它具有降低細(xì)胞增殖的能力，給細(xì)胞修復(fù)騰出時間。因此ATM一旦發(fā)生變異，功能失效可能導(dǎo)致免疫缺陷甚至是癌變。
    研究者認(rèn)為，Mre11不僅是DNA損傷的感受器，更是修復(fù)DNA的啟動因子，還能修飾受損的DNA分子