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磷酸化p53結合蛋白1抗體
  • 產品貨號：
    BN40845R
  • 中文名稱：
    磷酸化p53結合蛋白1抗體
  • 英文名稱：
    Rabbit anti-phospho-p53BP1 (Ser25 + Ser29) Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN40845R-100ul

    100ul

    ￥2470.00

    交叉反應：Mouse,Rat(predicted:Human,Dog,Pig,Cow,Horse,Rabbit) 推薦應用：IHC-P,IHC-F,ICC,IF,ELISA

產品描述

英文名稱phospho-p53BP1 (Ser25 + Ser29)
中文名稱磷酸化p53結合蛋白1抗體
別    名53BP1 (phospho S25); p-53BP1 (phospho S25) ;53BP1 (Phospho-Ser25/29); 53BP1 (Phospho-S25/S29); phospho-p53BP1(Ser25/Ser29); P-53BP1 (Ser25/29); 53 BP1; FLJ41424; MGC138366; p202; p53 binding protein 1; p53 BP1; p53BP1; TP53 BP1; TP53BP1; Tumor protein 53 binding protein 1; Tumor protein p53 binding protein 1; Tumor suppressor p53 binding protein 1; TP53B_HUMAN; tumor protein 53-binding protein, 1.  




產品類型磷酸化抗體 
研究領域腫瘤  細胞生物  染色質和核信號  信號轉導  激酶和磷酸酶  結合蛋白  表觀遺傳學  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, )
產品應用ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量213kDa
細胞定位細胞核 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human p53BP1 around the phosphorylation site of Ser25/29:ED(p-S)QPE(p-S)QV 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產品介紹p53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.

Function:
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.

Subunit:
Interacts with IFI202A. Binds to the central domain of p53/TP53. May form homooligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20' (H4K20me2). Has low affinity for histone H4 containing monomethylated 'Lys-20' (H4K20me1). Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20' (H4K20me3). Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro). Does not bind unmethylated histone H3. Interacts with MUM1/EXPAND1. Interacts with CHEK2; modulates CHEK2 phosphorylation at 'Thr-68' in response to infrared. Interacts with MSL1; this interaction may be required for MSL1 DNA repair activity, but not for histone acetyltransferase activity.

Subcellular Location:
Nucleus. Chromosome; centromere; kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.

Post-translational modifications:
Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.

DISEASE:
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.

Similarity:
Contains 2 BRCT domains.

SWISS:
Q12888

Gene ID:
7158

Database links:

Entrez Gene: 7158 Human

Entrez Gene: 27223 Mouse

Omim: 605230 Human

SwissProt: Q12888 Human

SwissProt: P70399 Mouse

Unigene: 440968 Human

Unigene: 383499 Mouse

Unigene: 481841 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

p53結合蛋白1(53BP1)是細胞周期檢查點激酶蛋白，是新近發(fā)現的DNA損傷修復通路中兩個非常重要的蛋白激酶MDC1和53BP1蛋白之一,目前多用于腫瘤方面的研究。






















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