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麥芽糖結(jié)合蛋白抗體
  • 產(chǎn)品貨號(hào)：
    BN40839R
  • 中文名稱：
    麥芽糖結(jié)合蛋白抗體
  • 英文名稱：
    Rabbit anti-MBP tag Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN40839R-100ul

    100ul

    ￥1360.00

    交叉反應(yīng)：nan(predicted:Escherichia Coli) 推薦應(yīng)用：WB,IHC-P,IHC-F,IF,ELISA

產(chǎn)品描述

英文名稱MBP tag
中文名稱麥芽糖結(jié)合蛋白抗體
別    名MBP-tag; MBP; MMBP; Maltose Binding Protein; Maltose binding protein tag; ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding protein; MALE_ECOLI.  
產(chǎn)品類型標(biāo)簽抗體 
研究領(lǐng)域細(xì)胞生物  免疫學(xué)  糖蛋白  
抗體來(lái)源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Recombinant protein
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量44kDa
細(xì)胞定位細(xì)胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from E. coli Maltose Binding Protein:1-100/396 
亞    型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

Function:
Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody.

Subcellular Location:
Periplasm.

Similarity:
Belongs to the bacterial solute-binding protein 1 family.

SWISS:
P0AEX9

Gene ID:
1039564

Database links:
Entrez Gene: 1039564 Escherichia coli

Entrez Gene: 4494002 Escherichia coli

Entrez Gene: 948538 Escherichia coli

Entrez Gene: 960089 Escherichia coli

SwissProt: P0AEX9 Escherichia coli

SwissProt: P0AEY0 Escherichia coli



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.